#31 - Pauline's Singing Debut

Donkey Kong's Fun With Music was a cancelled Famicom educational game. One of the few screenshots of it shows what would have been called the Donkey Band, with Pauline as a vocalist. This means Pauline's "career" as a singer began 34 years before Odyssey.

SOURCE: https://www.mariowiki.com/Donkey_Kong_no_Ongaku_Asobi